Nanodispersion of TiO2 in hypochlorous acid and its antimicrobial effect against oral pathogens
Resumen
El uso continuo e inadecuado de los agentes antimicrobianos tradicionalmente utilizados ha provocado el surgimiento de cepas bacterianas multirresistentes (MDR) y la mutación de microorganismos en el campo de la odontología. Por lo tanto, se han desarrollado varias nanopartículas para combatir patógenos resistentes. Las nanopartículas de dióxido de titanio (TiO2) han sido agentes antimicrobianos atractivos debido a su estabilidad química, no toxicidad y precursores económicos. Por lo tanto, las nanodispersiones basadas en TiO2 se exploraron preparándolas con agentes antimicrobianos bien conocidos, como el ácido hipocloroso (HOCl), para mejorar el efecto antimicrobiano. En este estudio, se sintetizaron y caracterizaron nanodispersiones de TiO2 NPs-HOCl basadas en sol-gel. El efecto antimicrobiano se evaluó mediante un ensayo de microdilución utilizando cepas de S. mutans, S. aureus, E. faecalis y C. albicans mediante la incubación de diferentes concentraciones de las nanodispersiones. Para evaluar los efectos citotóxicos, se inocularon células madre de la papila apical (SCAP) y se evaluaron mediante el ensayo MTT. La nanodispersión exhibió un efecto antimicrobiano mejorado, casi sin citotoxicidad. La nanodispersión basada en HOCl exhibió un mayor efecto antimicrobiano y alta estabilidad. Por lo tanto, se puede utilizar como un agente antimicrobiano prometedor para el tratamiento de diversos patógenos dentales.
Citas
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