Bismuth subsalicylate nanoparticles by laser ablation: effect against bacteria associated with nosocomial infections
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Abstract
Objective: to evaluate the antibacterial effect of bismuth subsalicylate nanoparticles (NPs-SSBi) against four bacteria, frequently associated with nosocomial infections. Methods: the NPs-SSBi were obtained in colloidal suspension by laser ablation of solids in liquids (ALSL). The size, composition, and stability of the NPs in suspension were analyzed by transmission electron microscopy and ultraviolet-visible spectroscopy. The planktonic growth and biofilm formation of two Gram-positive bacteria, S. aureus and S. epidermidis, and two Gram-negative bacteria, E. coli and P. aeruginosa, after exposure to different concentrations of NPs-SSBi (1.25 to 90 μg/mL), were evaluated by turbidity and XTT assays, respectively. Results: quasi-spherical crystalline NPs-SSBi were obtained, with a size of 4.5 ± 0.14 nm, which remain stable in colloidal suspension for at least 21 days. The NPs-SSBi inhibited the growth of all four bacteria, planktonic growth was reduced ≈80-92% at concentrations above 40 μL/mL, and biofilm formation ≈73-89% at concentrations of 80 and 90 μL/mL. Conclusions: the NPs-SSBi obtained by ALSL inhibited the growth of four important nosocomial bacteria, so they could be used for the control of health care-associated infections.
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Mundo Nano. Revista Interdisciplinaria en Nanociencias y Nanotecnología por Universidad Nacional Autónoma de México se distribuye bajo una Licencia Creative Commons Atribución-NoComercial 4.0 Internacional.
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