Dual effect of silver nanoparticles on Allium cepa root growth and Pseudomonas inhibition
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This study aimed to evaluate the effect of silver nanoparticles (AgNPs) on Allium cepa growth and their capacity to inhibit Pseudomonas species. The AgNPs were synthesized via a chemical reaction using sodium borohydride and sodium citrate, and characterized by X-ray diffraction, transmission electron microscopy (TEM), and UV-Vis spectroscopy. The AgNPs exhibited a cubic crystal structure, a 20 nm spherical shape, and a plasmonic resonance with a maximum at 400 nm. Bioassays were performed using onion roots exposed to varying AgNP concentrations, alongside bacteria of the Pseudomonas genus. The AgNPs at 5 mg/L promoted onion root growth, whereas concentrations above 50 mg/L inhibited root elongation. The 10% inhibitory concentration (IC₁₀ ) for root growth was 9 mg/L, and the median inhibitory concentration (IC₅₀) was 46.01 mg/L. For bacteria, the minimum inhibitory concentration (MIC) ranged from 5 to 10 mg/L for P. fluorescens, P. aeruginosa, and P. putida. These findings suggest that AgNPs can inhibit beneficial Pseudomonas species for plants but may also be used to control phytopathogenic bacteria with minimal inhibition on Allium cepa.
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Mundo Nano. Revista Interdisciplinaria en Nanociencias y Nanotecnología por Universidad Nacional Autónoma de México se distribuye bajo una Licencia Creative Commons Atribución-NoComercial 4.0 Internacional.
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